Bio-Rad Bio-Plex Pro™ Human Cancer Biomarker Assays - Manual (Page 30)

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Luminex xPONENT Software
Luminex xPONENT software may be used to analyze Bio-Plex assays.
Although guidelines are provided here, consult the xPONENT software
manual for more details. Perform a system initialization with Luminex’s
calibration and performance verification kit, as directed by Luminex.
Select Batches to set up the protocol and follow the information
under Settings.
Note! The instrument settings described below apply to Luminex 100/200
and FLEXMAP 3D instruments. For the Bio-Plex MAGPIX reader, use the
default instrument settings.
1. Select MagPlex as the bead type for magnetic beads. This
automatically sets the DD gates.
2. Volume = 50 µl.
3. Bio-Plex Pro human cancer assays are run at low PMT
(Standard
PMT).
4. Plate name: 96-well plate.
5. Analysis type: Quantitative, 5PL Curve Fit.
6. Number of standards: 8.
Select Analytes to set up the panel.
1. Enter “pg/ml” in the Units field.
2. Enter 50 in the Count field.
3. Select the bead region and enter the analyte name.
4. Click “Apply all” for Units and Count.
Select Stds and Ctrls.
1. Enter standard concentrations, lot number, dilution factor, and other
information as applicable.
After the assay is complete, select Results, then select Saved Batches.