Bio-Rad Model 550, Ultramark, and Benchmark Microplate Reader Accessories - Manual (Page 11)

4100172 Rev C 3
3. Using the Benchmark Plus
The Benchmark Plus is operated through the Protocol dialog boxes in Microplate Manager. See
the Microplate Manager User Guide for instructions on specifying the wavelengths selection and
operating the Benchmark Plus through the Protocol dialogs (Chapter 4).
3.1 Loading a Plate
To load a plate into the Benchmark Plus, press the Open/Close button on the front of the
instrument or click on the Reader Door: Open button in the Protocol dialog box in Microplate
Manager. The front door of the reader will open and the plate loader will extend outwards.
Place your microplate in the plate loader, pressing lightly to set the plate into place. Press the
Open/Close button again to close the reader door, or click on the Reader Door: Close button in
the Protocol dialog in Microplate Manager.
Ø The Benchmark Plus will accept any 6-well through 384-well plate with the dimensions 138
mm (length) x 98 mm (width). Plate height cannot exceed 15 mm.
3.2 Selecting a Wavelength in the Benchmark Plus
This section describes how to select the desired wavelength in the Benchmark Plus. Since
Benchmark Plus is a monochromator-based system, there shall be no need to physically change
any filter through hardware means.
Instead, all wavelength selections will be programmed with Microplate Manager (MPM)
software.
Start up the MPM software. Within the MPM, select the desired scan method. A window will
appear that allows the user to select either single or dual wavelength scanning. Within the
window any wavelength can be selected within the 340 to 800 nm range. See Section 4.2.3 in
Chapter 4 of the Microplate Manager User Guide for instructions.
3.3 Reading a Plate
Plate readings are controlled entirely by Microplate Manager. See Chapter 4 of the Microplate
Manager User Guide for instructions.
To stop a plate reading in progress, press the Stop button in the Microplate Manager Protocol
dialog box.