General information, 1 introduction, 2 ultramark specifications – Bio-Rad Model 550, Ultramark, and Benchmark Microplate Reader Accessories User Manual

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1. General Information

1.1 Introduction

The Ultramark microplate reader provides all the flexibility you need for virtually any absorbance
assay. You can use it to perform standard colormetric ELISA and kinetic readings for general cell
screening with 6, 12, 24, 96, 384, and 1536-well microplate formats. The Ultramark has a 10-magazine
filter holder with a working wavelength range of 260–750 nm and an incubation temperature range
up to 42 degrees Celsius. The optical design provides verifiable results up to 4.5 O.D. with an optical
resolution 500 microns.

1.2 Ultramark

Specifications

Light Source

Xenon Flash Lamp

Spectral Range

250–750 nm

Filter Capacity

10-position magazine

Shaking Yes

Incubation

Up to 42

°C , programmable in 0.2° C increments

Power

100–240 V, 60/50 Hz, 22 Amps

Dimensions

20 cm (H) x 43 cm (W) x 51 cm (D)

Compatible Plates

Any microplate or suitable medium with dimensions 138 mm
(length) x 98 mm (width). Plate height cannot exceed 15 mm.

Resolution 500

microns

Linearity

Less than 0.5% CV from 0–4.0 O.D. at 405 nm

Precision

Less than 0.5% CV from 0–4.0 O.D. at 405 nm

Software Compatibility

A System 486 or higher, 32 Mb RAM, Windows 95 OS or higher

Read Times

96-well plate:

6 seconds single wavelength

24

seconds

dual

wavelength

384-well plate:

12 seconds single wavelength

32

seconds

dual

wavelength

1536-well plate: 34 seconds single wavelength
78

seconds

dual

wavelength

Compatible Plate types

Any format from 1 well to 1536 wells, up to a plate height of 15
mm.

Environmental Operating
Range

5–40 degrees C; max. relative humidity 80% non-condensing

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