Bio-Rad Aminex Organic Acid and Alcohol Analysis Columns - Manual (Page 19)

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Section 8
Column Storage
Always be certain to exclude air when closing column end fittings. For prolonged
storage, the columns may be refrigerated to prevent drying out. However, they
should never be frozen. If the column will not be used for several days, it should be
stored according to one of these procedures.
8.1 Long Term Storage
1. Replace the mobile phase with the appropriate storage/shipping solvent as
shown in Table 2. The flow rate during the mobile phase change should be
kept lower than normal flow rate.
2. Keep the ends of the column tightly capped. Use the nuts originally furnished
with the column. This minimizes evaporation of solvent and keeps the resin
fully hydrated. The detector cells should be flushed as well.
3. Store the column in the original shipping container when not in use. To help
prevent evaporation and microbial growth, resin columns can be stored at
4°C (not frozen).
8.2 Short Term Storage
If the column is to be used daily, the eluant may be left in the column overnight.
Keep a low flow passing through the column to prevent buffer salt precipitation
and to allow faster equilibration the next day. If a halide salt is present in the mobile
phase, slowly rinse and store the column in distilled, deionized water or
appropriate storage solvent prior to storage.
Section 9
Troubleshooting
Table 3 is a troubleshooting guide, which also refers to regeneration procedures,
listed as Procedures 6.1–6.5. These procedures are given in detail in Section 6.
Operating the column within the guidelines given in Table 1 and Table 2 will help
maintain a long column lifetime. Any deviation from these guidelines can reduce
column efficiency and shorten column life.
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