Bio-Rad Profinity Epoxide Resin - Manual (Page 11)

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Section 7
Regeneration and Storage
Regeneration conditions depend on which type of ligand or protein is used in the
coupling and chromatography process. Refer to textbooks and literature articles
for guidance. In general, 2–4 wash cycles alternating acidic and basic buffers are
useful. Each cycle should consist of a wash of a pH 4.0 buffer (100 mM acetate,
500 mM NaCl) followed by a wash of a pH 8.0 buffer (100 mM phosphate,
500 mM NaCl).
Profinity epoxide resin should be stored dry between 4°C and ambient tempera-
ture. Ligand-coupled Profinity resin should be stored at 4–8°C in an appropriate
buffer that contains a bacteriostat.
Section 8
References
Frost RG et al., Covalent immobilization of proteins to N-hydroxysuccinimide ester
derivative of agarose. Effect of protein charge on immobilization, Biochim Biophys
Acta 670, 163–169 (1981)
Gagnon P, Purification Tools for Monoclonal Antibodies, Validated Biosystems,
Inc., Tuscon (1996)
Hermanson GT et al., Immobilized Affinity Ligand Techniques, Academic Press,
New York (1992)
Izuta S and Saneyoshi M, AraUTP-Affi-Gel 10: a novel affinity absorbent for the
specific purification of DNA polymerase alpha-primase, Anal Biochem 174,
318–324 (1988)
Matejtschuk P (ed), Affinity Separations — A Practical Approach, Oxford University
Press, Oxford (1997)
Section 9
Ordering Information
Ordering Information
Catalog # Description
156-0200 Profinity Epoxide Resin, 5 g
156-0201 Profinity Epoxide Resin, 25 g
Streptactin is a trademark of Institut für Bioanalytik GmbH. Triton is a trademark of Union Carbide.
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