Bio-Rad Profinity Epoxide Resin - Manual (Page 8)

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Recommended Columns
Bio-Rad’s Bio-Scale
™ MT high-resolution columns may be used for the above
column packing procedure. These columns are empty but may be packed with
the support of choice. Bio-Scale MT columns are convenient for use with
Bio-Rad’s BioLogic
™ system or with any medium- or high-pressure system.
• Bio-Scale MT2 column (7 x 52 mm) for bed volumes up to 2 ml
• Bio-Scale MT5 column (10 x 64 mm) for bed volumes up to 5 ml
• Bio-Scale MT10 column (12 x 88 mm) for bed volumes up to 10 ml
• Bio-Scale MT20 column (15 x 113 mm) for bed volumes up to 20 ml
Column packing — sample preparation sized columns
Use this method for packing Profinity resin into small micro spin columns for
sample preparation (such as Bio-Rad’s Micro Bio-Spin
™ chromatography
columns, empty, 100, catalog #732-6204).
1. Make a 50% slurry of ligand-coupled Profinity epoxide resin in degassed
binding buffer of choice. Resuspend the slurry by gently swirling or stirring
with a glass or plastic rod. Do not use a magnetic stirrer.
2. Place the column into an appropriate collection vessel (for example, a 2 ml
capless collection tube), and spin.
3. Using a pipet, transfer enough Profinity resin to a microcentrifuge tube. If
using Bio-Rad’s Micro Bio-Spin column, transfer ~0.2 ml slurried Profinity
resin to a Micro Bio-Spin column. This is equivalent to ~100 µl of a packed
resin bed.
4. Remove excess binding buffer by centrifugation. Centrifuge at 1,000 x g for
15 sec to pack resin. The column is now ready for separation.
Recommended columns for gravity-flow chromatography
The following Bio-Rad columns may be used for gravity-flow chromatography.
They are empty and can be filled with Profinity resin.
• Poly-Prep ® columns, for up to 2 ml chromatography support and 10 ml
sample
• Econo-Pac ® columns, for up to 20 ml
• Glass Econo-Column ® columns, from 5–170 cm long and 0.5–5.0 cm in
diameter
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