Bio-Rad Profinity Epoxide Resin - Manual (Page 3)

Section 1
Product Description
Profinity epoxide is an activated macroporous resin for the immobilization of
various ligands of interest. Ligands containing amino, thiol, or hydroxyl groups can
be coupled to Profinity epoxide through an epoxy ring-opening reaction under mild
conditions.
Profinity epoxide is based on Bio-Rad’s proprietary and innovative UNOsphere ™
technology (US patent 6,423,666). Resins made using this technology have
properties of superb mechanical strength, open pore structure, optimized ligand
density, and low, nonspecific binding effects. These unique features of the
UNOsphere base matrix enable ligand-coupled Profinity resin to exhibit excellent
flow properties and to perform separations at very fast flow rates without
compromising protein binding, recovery, or purity. Profinity resin’s open-pore
structure is particularly useful for the purification of large biomolecules.
The base matrix of Profinity epoxide resin is stable across the entire pH range
(1–14) and is compatible with most reagents commonly used in protein
purification, such as denaturing agents, detergents, and reducing agents. It is
amenable to separations under native or denaturing conditions using liquid
chromatographic instrumentation, gravity flow columns, or sample preparation spin
columns.
The resin is supplied dry and is available in 5 g and 25 g quantities.
Note: UNOsphere support, from which Profinity epoxide is derived, was designed
to achieve the highest productivity possible (as measured in grams of target
molecule per operational hour per liter of support). UNOsphere media may be run
at the highest rates and loading capacities while staying within the pressure limits
of the column and chromatography system.
1
UNOsphere
O OH
NHR (-SR, -OR)
R-NH
2
, -SH, -OH
UNOsphere
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