Bio-Rad Aurum™ Vacuum Manifold - Manual (Page 4)

Section 3
Care and Maintenance
The Aurum™ vacuum manifold should be stored clean and dry at room
temperature. After each use rinse with distilled water and wipe dry with
paper towels or air dry. Never use alcohol or other organic reagents to
clean the manifold. Never use abrasive materials to clean the manifold.
Gasket surfaces should be carefully rinsed to remove salts, which could
inhibit contact between manifold parts and the column adaptor plate
(CAP). Check the tubular and flat silicone gaskets for wear before and
after use of the manifold. In the event that the bottom tubular gasket
requires replacement, a length of
1/
8
" OD silicone tubing may be used.
2
Section 4
Aurum™ Vacuum Manifold
The Aurum vacuum manifold is designed to accommodate the 96-well plates
used in Bio-Rad's Aurum total RNA 96 kit. The manifold may also be
compatible with other manufacturers' kits. For column processing, the column
adaptor plate (CAP) is required.
The CAP allows the Aurum vacuum manifold to to be used for processing
columns with a luer fitting. The CAP has 18 black luer fittings in a 6 x 3 array.
Up to 18 Aurum miniprep columns can be accommodated on the CAP
without the need for connectors or other manifold accessories. The CAP will
also accommodate other columns with luer ends.
When vacuum is applied to the manifold, the CAP should self-seat, forming an
airtight seal without the need to press down on the CAP. However, the
application of gentle downward force may occasionally be required to facilitate
seating.
The vacuum regulator is included and strongly recommended for measuring
negative pressure, and for gradually increasing negative pressure during
different protocols.
The Aurum manifold consists of two primary parts: the manifold base and top.
The top has an attached tubular silicone gasket, allowing the manifold base
and top to seal when a vacuum is introduced. Attached to the opening in the
manifold top is a flat silicone gasket, enabling Aurum 96-well purification plates
or the CAP to establish an airtight seal with the manifold top when a vacuum
is applied. Collectively these two gaskets facilitate self-seating of all
components, reducing the need to apply external pressure to the manifold to
initiate vacuum. However, under certain conditions application of gentle
downward force on the plates or CAP may be required to facilitate seating. In
the event that the tubular silicone gasket becomes detached, it can easily be
reinserted into the track on the underside of the manifold top.
Each Aurum vacuum manifold is supplied with a stage that centers and
elevates a 96-well sample collection plate during elution from the purification
plate. The stage should be placed with the printed "this side up" in the base
with the groove side down to allow for airflow.
3
Fig. 1. Aurum vacuum manifold kit components.
Manifold top Manifold base with
quick connect fitting
Vacuum regulator
A stage
Luer caps Column adaptor plate (CAP) Tubing ( 5/
16
” ID)
Stage Waste collection tray