Bio-Rad Aurum™ Vacuum Manifold - Manual (Page 6)

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Section 8
Use for the Column Adaptor Plate
(CAP)
Manifold wash setup (see Figure 5)
1. Insert the CAP (luer ends up) into the depression in the vacuum manifold
top. Ensure that the CAP rests evenly on the gasket.
2. Insert the luer ends of the desired columns into the available luer fittings,
ensuring a tight fit.
3. Close the unused luer fittings with the caps provided. Close caps by
rotating clockwise until light resistance is encountered. Excessive
tightening of a cap may cause the luer fitting to dislodge when the cap is
removed.
4. The manifold is now ready for column processing according to the
vacuum protocol of the appropriate column purification kit.
5. When ready to elute, proceed with the appropriate spin elution step as
recommended by the protocol.
6. After finishing the elution, rinse the CAP and Aurum vacuum manifold
with water, and air dry or wipe with paper towels.
Fig. 5. Setup and use for column processing.
Section 7
Use for 96-Well Plates
Manifold wash setup (see Figure 4A)
1. Place the manifold top on the base, ensuring complete and uniform
contact between the manifold top and base.
2. Place the desired 96-well purification plate on the manifold top and apply
the recommended vacuum pressure for your application.
Manifold elution setup (see Figure 4B)
3. When eluting from the purification plate, place the stage on the manifold
base, with the etched "this side up" facing up. Place a clean 96-well
microtiter collection plate securely on top of the stage and replace the
manifold top.
Note: If desired, waste can be collected into an optional pipet tip box lid
instead of draining directly into the vacuum trap.
4. After use, rinse the Aurum™ vacuum manifold with water and air dry or
wipe with paper towels. Failure to rinse the vacuum manifold at the end of
each use will cause the manifold to become cloudy and pitted.
Fig. 4. Setup and use for 96-well plate processing.
A) 96-well wash; B) 96-well elution.
A. B.