Bio-Rad PureZOL™ RNA Isolation Reagent - Manual (Page 5)

Section 2
Materials and Equipment Required
(Not Provided)
Microcentrifuges (capable of >12,000 x g) — at 4°C and at room
temperature
95–100% ethanol, ACS grade or better
Chloroform (without additives such as isoamyl alcohol); need
0.2 ml of chloroform per 1 ml of PureZOL
Isopropyl alcohol; need 0.5 ml per 1 ml of PureZOL
RNase-free water (DEPC-treated water)
75% ethanol (prepared in DEPC-treated water); need 1 ml per
1 ml of PureZOL
RNase-free pipet tips and micropipets
RNase-free polypropylene centrifuge tubes with caps, capable of
withstanding centrifugal forces of 12,000 x g
Disposable latex or vinyl gloves
Supplies for tissue grinding, disruption, and homogenization
• Fresh tissue — tissue cutter and a ruler to measure size
• Frozen tissue — liquid nitrogen and mortar and pestle
• Tissue homogenizer — dounce homogenizers, rotor-stator
homogenizers, or bead mill homogenizers are recommended
Optional — Aurum total RNA mini kit (732-6820) for RNA cleanup
following isolation of RNA using PureZOL (see section 8 for order-
ing information)
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