Bio-Rad PureZOL™ RNA Isolation Reagent - Manual (Page 7)

Solutions that are prepared by the user should be treated with
DEPC to inactivate RNases. See instructions below on how to
prepare DEPC-treated solutions.
Keep reagent bottles closed when not in use and keep RNA
samples on ice to prevent degradation by RNases.
Preparing Reagents Not Supplied With the Kit
Note: DEPC is destroyed by primary amines. If a solution
containing a primary amine will be DEPC-treated, omit the amine
in preparing the solution. Perform the DEPC treatment as
described above, and add the amine to the autoclaved solution
once the solution has cooled.
Preparation of DEPC-treated water. To prepare a 0.1% (v/v)
solution of DEPC-treated water, add 1.0 ml of liquid DEPC per
1 L of water. Incubate the solution at 37°C for 1 hour while mixing
thoroughly. Autoclave the treated water to remove the DEPC.
Preparation of 75% ethanol. To prepare 100 ml of 75% ethanol,
add 75 ml of 95–100% ethanol to 25 ml of DEPC-treated water.
Mix well before use.
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