Bio-Rad Protein Assay User Manual
Page 12
Table 2. Comparison of Bio-Rad, Lowry, and Biuret Protein
Assays for 23 Proteins
All assays were performed on appropriate dilutions of gravimetrically pre-
pared 10 mg/ml solutions of commercially obtained proteins. Standards
were as described in the text.
Assay Results (Protein mg/ml)
Biuret
Lowry
Bio-Rad
1.
Alcohol dehydrogenase
5.8
5.0
7.8
2.
α
-Amylase
6.8
6.0
8.3
3.
Bovine serum albumin
9.7
8.4
21.1
4.
Carbonic anhydrase
8.8
8.9
13.0
5.
Catalase
7.6
6.3
9.7
6.
α
-Chymotrypsin
9.4
11.6
7.8
7.
Cytochrome c*
25.7
11.3
25.3
8.
Ovalbumin
10.2
10.1
9.4
9.
Fibrinogen
6.2
7.3
7.8
10.
Gamma globulin (rabbit)
9.4
11.8
8.0
11.
β
-Galactosidase
9.5
9.9
7.9
12.
Hemoglobin (bovine)*
16.2
8.3
19.9
13.
Histones
9.7
9.2
15.8
14.
Hemocyanin
6.6
5.4
9.2
15.
Lysozyme
10.4
12.6
9.9
16.
Myoglobin*
13.7
7.9
20.7
17.
Ovomucoid
7.8
8.3
5.9
18.
Pepsin
9.8
12.4
4.1
19.
Ribonuclease
11.8
15.9
5.3
20.
Trypsin inhibitor (soy)
9.1
10.3
6.1
21.
Transferrin
8.5
9.0
12.6
22.
Trypsin
11.4
15.5
4.9
23.
Thyroglobulin
7.7
8.2
9.3
Average
10.1
9.5
10.9
*Average
8.8
9.6
9.2
*
The second average eliminates from the figures the values for the three colored proteins shown by
asterisks.
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