Bio-Rad Protein Assay - Manual (Page 4)

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Table 1. Reagents Compatible with the Bio-Rad Protein Assay
When Using the Standard Procedure.*
Acetate, 0.6 M KCI, 1.0 M
Acetone Malic acid, 0.2 M
Adenosine, 1 mM MgCl
2
, 1.0 M
Amino Acids Mercaptoethanol, 1.0 M
Ammonium sulfate, 1.0 M MES, 0.7 M
Ampholytes, 0.5% Methanol
Acid pH MOPS, 0.2 M
ATP, 1 mM NaCl, 5 M
Barbital NAD, 1 mM
BES, 2.5 M NaSCN, 3 M
Boric acid Peptones
Cacodylate-Tris, 0.1 M Phenol, 5%
CDTA, 0.05 M Phosphate, 1.0 M
Citrate, 0.05 M PIPES, 0.5 M
Deoxycholate, 0.1% Polyadenylic acid, 1 mM
Dithiothreitol, 1 M Polypeptides (MW<3000)
DNA, 1 mg/ml Pyrophosphate, 0.2 M
EDTA, 0.1 M rRNA, 0.25 mg/ml
EGTA, 0.05 M tRNA, 0.4 mg/ml
Ethanol total RNA, 0.30 mg/ml
Eagle’s MEM SDS, 0.1%
Earle’s salt solution Sodium phosphate
Formic acid, 1.0 M Streptomycin sulfate, 20%
Fructose Triton X-100, 0.1%
Glucose Tricine
Glutathione Tyrosine, 1 mM
Glycerol, 99% Thymidine, 1 mM
Glycine, 0.1 M Tris, 2.0 M
Guanidine-HCI Urea, 6 M
Hank's salt solution Vitamins
HEPES buffer, 0.1 M
* Interference may be caused by chemical-protein and/or chemical-dye interactions. Table 1 lists
those chemical reagents not directly affecting the development of dye color. Since every protein-
chemical reagent combination has not been assayed, it is possible that some of the listed reagents
produce interference in combination with certain proteins. However, with respect to proteins such as
bovine albumin and globulin, the above listed reagents show little or no interference.
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