Labconco Biological Safety Cabinets 34269 Series - Manual (Page 49)

Chapter 5: Using The Cabinet
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Original instructions
Loading Materials and Equipment
• Only load the materials required for the procedure. Do not overload
the cabinet.
• Do not obstruct the front, side, or rear return air grilles.
• Large objects should not be placed close together.
• Slowly close the sash until it is in the correct operating position.
• After loading the cabinet, wait two to three minutes to purge airborne
contaminants from the work area.
Work Techniques
• Keep all materials at least 4 inches (100 mm) inside from the sash,
and perform all contaminated operations as far to the rear of the work
area as possible.
• Segregate all clean and contaminated materials in the work area.
• Arrange materials to minimize the movement of contaminated
materials into clean areas.
• Keep all discarded contaminated material to the rear of the work area.
• Avoid moving materials or the operator's hands and arms through the
front access opening during use.
• Avoid the use of an open flame. Use disposable labware or an electric
incinerator as alternatives.
• Use proper aseptic technique.
• Avoid using techniques or procedures that disrupt the airflow patterns
of the cabinet.
• If there is a spill or splatter during use, all objects in the cabinet
should be surface decontaminated before removal. Thoroughly
disinfect the working area of the cabinet WHILE IT IS STILL IN
OPERATION, to prevent the release of contaminants from the
cabinet.
Final Purging
• Upon completion of work, the cabinet should be allowed to operate
for two to three minutes undisturbed, to purge airborne contaminants
from the work area.