Labconco FreeZone 1 Liter Benchtop Freeze Dry Systems 7740071 - Manual (Page 25)

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Chapter 4: Using Your Freeze Dryer
Product Service: Domestic 1-800-522-7658, International 816-333-8811
Pre-Freezing Samples
Appropriate containers for freeze drying include ampules, serum
bottles, and wide mouth freeze drying flasks. Shell freezing of
samples is recommended for wide mouth freeze drying flasks.
Smaller samples in ampules and serum bottles may be frozen in a
freezer. The sample container size should always be at least two to
three times the sample size (i.e., 40 ml samples should be prepared
in 80 ml containers or larger). The temperature required for pre-
freezing is dependent on the characteristics of the sample. Pre-
freezing temperature typically is at least 10° to 20°C below the
eutectic or collapse temperature of the sample.
Adding Sample
The following procedure should be followed when using sample
valves in the freeze dry process:
1. Pre-freeze samples. Appropriate containers for freeze drying include
ampules, serum bottles, and wide mouth freeze drying flasks. Shell
freezing of samples is recommended for wide mouth freeze drying
flasks. Smaller samples in ampules and serum bottles may be frozen
in a freezer or in the center of the collector chamber. The sample
container size should always be at least two to three times the sample
size (i.e., 40 ml samples should be prepared in 80 ml containers or
larger).
2. Connect a pre-frozen sample to a sample valve on the drying chamber
or manifold using an adapter. Turn the plastic valve knob to the
“VACUUM” position to open the valve, which connects the attached
sample to system vacuum. The bevel on the knob should be
positioned toward the sample port to apply vacuum to the sample.
Bevel in “vent” position
Vent/Backfill Port
Bevel in “vacuum”
position
Adapter