Labconco Precise HEPA-Filtered Glove Boxes 5220231 - Manual (Page 26)

Chapter 5: Using Your Glove Box
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Loading Materials and Equipment (see Figure 5-1)
• Load only the materials required for the procedure. Do not overload
the glove box.
• Be aware that gloves will inflate when the glove box is operated
under negative pressure. Ensure items in the glove box will not
obstruct the movement of the gloves.
• After loading, wait 2-5 minutes at 50-100% blower speed to purge
airborne contaminants from the work area before beginning
procedures.
Work Techniques
• Segregate all clean and contaminated materials in the work area.
Final Purging (see Figure 5-1)
• All open trays, weigh vessels or containers must be sealed before
being removed from the glove box.
• Objects in contact with contaminated material must be surface
decontaminated before removal from the glove box.
• The interior surfaces should be cleaned per user’s protocol.
• Upon completion of work, the glove box should be allowed to operate
for 5 minutes undisturbed at 100% speed or 10 minutes at 50% speed
to purge and dilute airborne contaminants from the work area.
• After purging the main chamber, the transfer chamber should also be
purged for 5 minutes at 100% speed or 10 minutes at 50% speed with
the inner door open, before removing sealed material.
Shutdown
• Turn off the exhaust system and light.
• Keep outer door closed when not in use.