Iso class 3 definition, Chapter 2: prerequisites, Table 2-1 iso classification number (n) – Labconco Precise HEPA-Filtered Glove Boxes 5220231 User Manual

Page 9

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Chapter 2: Prerequisites

Product Service 1-800-522-7658

5

Precise HEPA-Filtered Glove Box Particle Testing under Positive Pressure

0

139

18

8

0

0

306

12

2

1

0

0

50

100

150

200

250

300

350

400

450

500

0

1

2

3

4

5

6

Time (Minutes)

N

u

m

b

er

o

f P

ar

tic

le

s

(P

er

C

u

b

ic M

ete

r P

er

M

in

u

te)

> 0.3 Micron

> 0.5 Micron

Airflow Testing Performed at 50 Dial Setting (61 CFM)

with Glove Box Under Positive Pressure.

NOTE: Ambient Air Baseline Particle Count at 1 Minute
198671 Particles measuring 0.3 Micron detected within 1 Minute.
25473 Particles measuring 0.5 Micron detected within 1 Minute.

ISO Class 3 Definition

Airborne particulate cleanliness inside any clean air glove box or enclosure is
designated by ISO Class 3, which is equivalent to 35 particles 0.5 µm or
larger per cubic meter of air per minute as defined by ISO Standard 14644-1.
ISO Class 3 cleanliness is illustrated in the table below and is equivalent to
Class 1 air conditions as defined by Federal Standard 209E or l particle 0.5
µm or larger per cubic foot of air per minute.

Table 1-1 Selected airborne particulate cleanliness classes for cleanrooms and clean zones.

ISO

classification

number (N)

Maximum concentration limits (particles/m

3

of air/minute) for particles equal to

and larger than the considered sizes shown below (concentration limits are
calculated in accordance with 3.2 of Standard 14644-1)

0.1 µm

0.2 µm

0.3 µm

0.5 µm

1 µm

5 µm

ISO Class 1

10

2

ISO Class 2

100

24

10

4

ISO Class 3

1 000

237

102

35

8

ISO Class 4

10 000

2 370

1 020

352

83

ISO Class 5

100 000

23 700

10 200

3 520

832

29

ISO Class 6

1 000 000 237 000

102 000

35 200

8 320

293

ISO Class 7

352 000

83 200

2 930

ISO Class 8

3 520 000

832 000

29 300

ISO Class 9

35 200 000

8 320 000

293 000

Table 2-1 ISO Classification Number (N)

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