Luminex xPONENT 3.1 - Manual (Page 49)

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Touring the Software
NOTE: Gate positions are dependent upon buffer composition. Any
changes made to your assay buffer composition may result in a
different optimal gate location.
Right-click inside the histogram area to access the following options:
X Axis - Use this to change the X axis to the following: Doublet Discriminator, Reporter 1,
Classification 1, and Classification 2. Doublet Discriminator is the default.
NOTE: You can change the X axis of the histogram for troubleshooting
purposes. Use only the default settings in all other instances.
Gate - Show, create, delete, or move the gate. You can also move the gates by clicking
and dragging the red lines on the histogram.
Autoscale - Click to automatically adjust the maximum number of events shown on the Y
axis. Click during acquisition to readjust the Y axis scale.
Set Scale - The Set Scale dialog box displays. Set the maximum number of events and
click OK. The default number of events is 60.
View - You can choose to view all events, or only gated events.
Display Mode - Opens the Histogram Options dialog box. Click either Logarithmic or
Linear display. Logarithmic display is the default. Select the Zoom check box for a
magnified view of the histogram.
Log. Displays a log of system processes. The log includes the following information:
• Date
• Message
• Code
Log entries indicating warnings are highlighted yellow. Errors are highlighted in red. Other
log entries are not highlighted. Click the maximize button to maximize the log. The log
expands to fill the whole window. Click minimize to return to the standard size.
Save Image. Opens a Save As dialog box to save a screen capture.
Details. Opens the Results tab, enabling more analysis and results. For more
information, see “Results Tab” on page 41.
Progress. Click to display real-time progress of the well acquisition. Analyte counts are
displayed in a dynamic bar graph as they are acquired. The scroll bar at the bottom of the
Progress display scrolls through the analyte list.
• Maximize - Maximizes the Progress display to fill the window.
• Zoom - Enable a closer look at the analyte progress.
• Save Image - Opens a Save As dialog box to save a screen capture.
• Default - Returns to the histogram and dot plot display.
Well Report. Displays a representation of the plate and the status of acquired wells. Wells
will display one of three possible states: