Luminex xPONENT 3.1 - Manual (Page 76)

Luminex xPONENT 3.1 Software Manual
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• Daily Instrument Shutdown (Luminex)
• Daily Instrument Startup (Luminex)
• Fluidics Maintenance (Luminex)
• None
• Prepare for Storage (Luminex)
• Remove Air Bubbles (Luminex)
• Remove Clog (Luminex)
• Revive After Storage (Luminex)
• Sanitize (Luminex)
• Weekly Maintenance (Luminex)
Commands. This section contains the following commands:
• Wash - Sends distilled water through the fluidic lines in the system. It pulls the fluid
from a well or from the reservoir and runs it completely through the system to the waste
receptacle.
• Soak - Prevents salt crystals from forming in the probe due to air exposure. Soaking
the probe replaces sheath fluid in the probe with water. You should perform the soak
function at the end of each day. The system uses at least 250 µL of distilled water.
• CAL1 - Calibrates DD, CL1, and CL2 for original non-magnetic xMAP beads.
• CON1 - Verifies DD, CL1, and CL2 for non-magnetic xMAP beads.
• Alcohol Flush - Removes air bubbles from the sample tubing and the cuvette using
70% isopropanol or 70% ethanol. The alcohol flush takes about five minutes. Alcohol
Flush uses the Luminex XYP reservoir because only the reservoir can accommodate
the amount of fluid necessary to flush the instrument.
• Warmup - Warms the system to prepare the optics prior to sample acquisition. The
system automatically begins warming up when you turn power on. The process takes
thirty minutes. You will need to use the Warmup command if the system is idle for four
hours or longer.
• MagCAL1 - Calibrates DD, CL1, and CL2 for MagPlex beads.
• MagCON1 - Verifies DD, CL1, and CL2 for MagPlex beads.
• Sanitize - The sanitize command performs a similar function as the alcohol flush
command, but uses 10% to 20% household bleach and water solution to
decontaminate sample lines and the cuvette after biohazard contact. Sanitize as part of
your daily shutdown routine after biohazard contact.
• Prime - Removes air from the system’s fluidic pathways by drawing sheath fluid from
the sheath fluid container. You do not need to supply solution in a plate.
• CAL2 - Calibrates xMAP reporters (RP1).
• CON2 - Verifies RP1 at normal and high PMT for all xMAP bead types.
• Drain - Helps remove debris from the bottom of the cuvette during troubleshooting.
You do not need to supply solution. Draining takes approximately two minutes and
should be followed by an alcohol flush with 70% isopropanol or 70% ethanol. Any fluid
that drains from the system drains to the Luminex XYP reservoir as the default.
However, you can set the system to drain to any unused well on the microtiter plate.
The drain function normally expels 125 µL of fluid.
• Backflush - Removes obstructions from the fluidics pathways by drawing sheath fluid
from the sheath fluid container. You do not need to supply solution.
• Self Test - Performs a self-diagnostic to see if the system and all operations are
functioning correctly. Self Test checks the following: