Multichannel Systems MEA Manual - Manual (Page 25)

MEA Manual
25
4.13 MEA with 9 Wells for Use with USB-MEA256-System
256-9wellMEA300/30iR-ITO
The
256-9wellMEA300/30iR-ITO has 256 electrodes and has to be used with the USB-MEA256-System.
Please refer to the USB-MEA256-System manual for detailed information.
The dimension of the glass carrier is 49 x 49 x 1 mm as usual. The MEAs with 9 wells are stable
in a temperature range from 0 ° - 125 °C.
The
256-9wellMEA contains 234 recording, 18 stimulation or recording, and four ground electrodes
arranged in nine electrode blocks with a 6 x 5 layout grid for the recording electrodes, two stimulation
or recording electrodes, and one reference electrode per each block. They are embedded in a transparent
glass substrate. The contact to the amplifier is provided by a double ring of contact pads around the rim of
the MEA. The standard material for MEAs is also used for
256-9wellMEA : The electrodes are from titanium
nitride (TiN) with a silicon nitride (SiN) isolator, and contact pads and tracks are made of transparent indium
tin oxide (ITO).
Using the
256-9wellMEA with macrolon quadrate, you have nine separate culture chambers on one MEA,
for example, for drug application in a screening experiment.
The spacing between the recording electrodes in the 6 x 5 grid averages 300 μm between the electrodes.
The electrode diameter of 30 μm results in an impedance of approximately 30 - 50 k. The dimension of
the square stimulation electrode is 50 x 200 μm.
Using 256-9wellMEAs
The same material is used for standard MEAs and 256-9wellMEAs . Therefore, they are equally robust and
heat-stabilized. They can be autoclaved and coated with different procedures for cell and tissue cultures.