Multichannel Systems MEA Manual - Manual (Page 45)

MEA Manual
45
5.5.8 MEA Cleaning with Terg-A-Zyme
Materials:
 Terg-A-Zyme (Sigma-Aldrich, Inc., Z273287)
 Distilled water
Terg-A-Zyme solution:
 Prepare a 1 % solution of Terg-A-Zyme in distilled water.
Method:
1. Place the MEA in 1 % Terg-A-Zyme solution overnight at room temperature.
2. Apply gentle shaking or rocking, if possible.
3. After Terg-a-Zyme treatment, rinse the MEA thoroughly with distilled water. (Terg-A-Zyme solution
can be stored at 4 °C and reused for about a week).
4. Dry the MEA and apply hydrophilic surface treatment, if necessary (Please see above).
5. If the MEA is going to be used for cell or tissue culture, autoclave the MEA at 121 °C for 30 min.
6. Do not fix cells or tissues on a MEA. Detergent treatment will not remove fixed tissues.
Important: NEVER wipe the electrode field or touch it otherwise!
Warning: It is absolutely necessary to rinse the MEAs thoroughly with distilled water after treatment
with detergent, particularly when using Terg-A-Zyme before heat sterilization (dry-heat sterilization
is not recommended). Otherwise the potential rests of the detergent may burn into the glass carrier
of the MEA and may destroy the electrodes.