Multichannel Systems MEA Manual - Manual (Page 28)

MEA Manual
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4.16 Square MEA: 60SquareMEA
60SquareMEA200/50iR-Ti
60SquareMEAs have 60 electrodes in an 8 x 8 layout grid with square electrode of 50 x 50 μm size and
interelectrode distances of 200 μm. They are available with an internal reference electrode. You can
connect the internal reference electrode directly to the amplifier's ground and will not need silver pellets
for grounding the bath anymore.
The flat, square electrodes are made of titanium nitride (TiN). The electrode size of 50 x 50 μm guarantees
very low noise. MEAs with TiN electrodes are very stable. Therefore, the MEAs can be reused several times
and are perfect for long-time experiments (up to several weeks and even months). The electrode impedance
ranges about 30 k
Tracks are made of titanium (Ti) and contact pads are made of titanium nitride (TiN); insulation material
is silicon nitride.
Important: MEAs are not symmetrical! That is, why the writing (for example NMI, LEITER, MEA type) on
the MEA chip should be on the right side viewed from the front, with the sockets of the amplifier in the
back. MEAs with one big internal reference electrode should be placed with reference electrode to the left
side in the amplifier. Otherwise, the MEA layout will not match with the pin layout of the channel map in
MC_Rack.
Using 60SquareMEAs
MEAs with square electrodes can be used for a wide variety of applications. They are robust and heat-
stabilized. They can be autoclaved and coated with different procedures for cell and tissue cultures.
Generally, they can be used for acute experiments as well as long-term cultures.