Multichannel Systems MEA Manual - Manual (Page 26)

MEA Manual
26
4.14 120MEA with for Use with MEA2100-120-System
120MEA200/30iR-Ti, 120MEA100/30iR-Ti, 120pMEA200/30iR-Ti
120MEA200/30iR-Ti
120pMEA200/30iR-Ti (electrode field with perforation)
The
120MEA200/30iR-Ti has 120 electrodes and can only be used with the MEA2100-System connected
to a headstage HS120 with 120 electrodes. Please refer to the MEA2100-System manual for detailed
information.
The dimension of the glass carrier is 49 x 49 x 1 mm as usual. The MEAs with 120 electrodes are stable
in a temperature range from 0 ° - 125 °C.
The
120MEA200/30iR-Ti contains 120 recording, four reference and four ground electrodes arranged in
a 12 x 12 layout grid. They are embedded in a transparent glass substrate. The contact to the amplifier is
provided by a double ring of contact pads around the rim of the MEA. The standard material for MEAs is
also used for
120MEA200/30iR-Ti: The electrodes are from titanium nitride (TiN) with a silicon nitride (SiN)
isolator, and contact pads and tracks are made of titanium nitride (TiN).
The spacing between the recording electrodes in the 12 x 12 grid averages 100 or 200 μm between
the electrodes. The electrode diameter of 30 μm results in an impedance of approximately 30 - 50 k.
This MEA is also available as perforated 120pMEA200/30iR-Ti. The electrodes are from Titan Gold Titan,
all other MEA parameters are the same. The inner diameter around the electrodes of 3 to 4 mm
2
is perforated and the total area of holes is 12 % of these area.
Using 120MEAs
The same material is used for standard MEAs and 120MEA200/30iR-Ti and 120MEA100/30iR-Ti . Therefore,
they are equally robust and heat-stabilized. They can be autoclaved and coated with different procedures
for cell and tissue cultures.
Using 120pMEA200/30iR-Ti
Perforated MEAs have a glass carrier, but the electrodes are embedded in polyimide foil. Therefore they
are heat stable to 50 °C only, and cannot be autoclaved. Please do not use an ultrasonic bath for cleaning.