Checking the volume – BrandTech Scientific Transferpette S User Manual

Page 35

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English

Depending on use, we recommend inspection of the instrument every
3 to 12 months. The cycle can, however, be adjusted to individual
requirements.
The gravimetric testing of the pipette volume is performed according to
the following steps and is in accordance with DIN EN ISO 8655, Part 6.

1.

Set nominal volume

Set volume to the maximum volume indicated on the instrument (see
page 32 for procedure).

2.

Condition the pipette

Condition the pipette before testing by using a pipette tip to aspirate and
discharge the test liquid (distilled H

2

O) five times. After this, discard the

pipette tip.

3.

Carry out the test

a) Aspirate liquid and pipette it into the weighing vessel.
b) Weigh the pipetted quantity with an analytical balance. (Please follow

the operating manual instructions from the balance manufacturer.)

c) Calculate the volume, taking the temperature of the test liquid into

account.

d) At least 10 pipettings and weighings in three volume ranges

(100%, 50%, 10% of nominal volume) are recommended for
statistical analysis.

Checking the Volume

According to DIN EN ISO 8655-2, it is recommended to replace the
tip after each measurement. This rule may be waived in accordance
with the DAkkS guideline DKD-R8-1.

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