UVP ColonyDoc-It Imaging Station - Manual (Page 15)

ColonyDoc-It Imaging Station 15
Lighting Tips:
There are four options for lighting the Petri dish. Epi Lighting options include White light and Blue light and
these sources light the Petri dish from the top. Base Lighting options include Trans and Darkfield and
these sources light the Petri dish from the bottom
Blue light is used to excite GFP stained colonies.
A majority of samples prepared using the spread method and a transparent substrate that are bacterial,
yeast, or mold are best viewed using Base Lighting > Trans.
If there is excessive handwriting on the bottom of the Petri dish, membrane paper, or stickers, then use Epi
Lighting > White or Base Lighting > Darkfield.
Use Base Lighting > Darkfield when counting a pour plate.
Using the black plate on top of the transillumination surface in combination with Epi Lighting sources
provides contrast that improves colony counting results.
Preparing Samples:
When preparing samples, write on the bottom edge away from the center of the Petri dish to increase
counting accuracy.
Automatic Counting
Before proceeding through the automatic counting steps, ensure that an image of the Petri dish has been captured
and is ready for analysis. See instructions on Quick Capture in this manual for more information on how to capture
an image.
Select Start Colony Count along the
left side of the screen. A new Colony
Count Type Selection window
appears. Select Automated Count
and OK.