Metrohm 871 Advanced Bioscan - Manual (Page 31)

4.2 Practical notes on ion chromatography
871 Advanced Bioscan / Instructions for Use 8.871.1003
23
fices for the analysis problems at hand. Compare the standard chro-
matogram enclosed with the column with your own measurements. If
any difficulties arise, you should always first check the quality of the
column by recording a standard chromatogram.
Yo
rm i ns
availa aflets su ation
Work otes, which are available on request free of charge at
your local Metrohm agency.
Prote
To
t foreign particles which could have an
adverse influence on the separation efficiency, we advise you to subject
both the eluents and all samples to microfiltration (0.45 µ m filter) and
to siphon the eluent through the 6.282
To av
ive particles arising from piston seals
of the sure pump, an inline filter should be installed (directly
after the pump). The 6.2821.120 Filter unit PEEK is best suited for
this purpose. It is a part of the standard equipment of the 818 IC Pump.
Storage
Wh
he sep d
in turer’s
Regeneration
If the separation properties of the column have deteriorated, it can be
u will find additional detailed info
ble from Metrohm in the le
s and -N
at on on the separating colum
pplied and in the Applic
ction
protect the column agains
1.090 Aspirating filter.
oid contamination by abras
high-pres
en not in use, always store t
accordance with the manufac
arating columns sealed and fille
specifications.
regenerated in accordance with the column manufacturer’s specifica-
tions. With the separating columns available from Metrohm, the instruc-
tions for regeneration can be found on the leaflet enclosed with every
column.
In the case of separating columns with carrier material based on silica,
only solutions with pH 2 … 7 may be used for regeneration, other-
wise the columns could be damaged.
ssure pump 4.2.2 High-pre
P
F
p needed. Metrohm
recommends the use of the 6.2620.150 Pulsation dampener MF. It is
lso used to protect the column material against pressure shocks
caused by the injection. Its installation is described in section 2.4.3.
Maintenance
To protect the pump against foreign particles, we advise you to subject
the eluent to microfiltration (0.45 µ m filter) and siphon the eluent
through the 6.2821.090 Aspirating Filter.
In many cases, an unstable baseline (pulsation, flow fluctuations) can
be traced to contaminated valves or faulty, leaky piston seals.
ulsation dampener
or determinations using the Advanced Bioscan pulsation-free high-
ressure pumps with very constant flow rates are
a