Metrohm 850 Professiona IC Cation Prep 1 - Manual (Page 20)

2.3 Flow diagram
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850 Professional IC –Cation – Prep 1
8 Install sample path connections.
￭ Connect the sample degasser (if required) (see Chapter 2.13,
page 42).
￭ Installing sample preparation (see Chapter 2.3, page 10).
￭ Connect sample path connections to the injection valve (see
Chapter 2.15.2, page 49).
9 Connect detector capillaries (see Chapter 2.17, page 53).
10 Mains connection (see Chapter 2.18, page 55).
11 Connect the instrument to the PC (see Chapter 2.18.1, page 55).
12 Initial start-up (see Chapter 3.1, page 60).
13 Install the guard column (if used) (see Chapter 2.19, page 56).
14 Install the separation column (see Chapter 2.20, page 58).
2.3 Flow diagram
Figure 2 Flow diagram with dialysis and Figure 3 Flow diagram with
ultrafiltration show the flow paths for two with the 2.850.1030 perform-
able inline sample preparation technologies. The arrangement of the mod-
ules in the diagram corresponds to the front view of the instrument. Liq-
uid containers (eluent bottle, sample vessel, waste container, auxiliary sol-
utions container) and guard column (see Chapter 2.19, page 56) are not
shown in the diagram. Which pressure screws, connections and couplings
are used, is described in the installation chapters of the individual mod-
ules.
Dialysis
This inline sample preparation technology is applied for samples with
strong matrices, e. g. emulsions, samples containing fat and protein, body
fluids or heavily contaminated waste waters. The dialysis effectively
removes particles and colloids, thus preventing damage to the separation
column.
The driving force in dialysis is diffusion. Only ions can pass through the
dialysis membrane. In contrast to filtration, the membrane also holds back
other substances during dialysis, e. g. drops of oil, thus protecting the sep-
aration column against them.