Fluorescence microscope, The cassette fixture, The run key and led indicator – ChemoMetec NC-200 User Manual

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2 Cell counting with the NucleoCounter NC-200

991-0203 Rev. 1.1 Sep. 2012 6

2.1.1

Fluorescence microscope

The NucleoCounter NC-200 is a fluorescent microscope with a magnification of

approximately x1.3. It is equipped with 3 light sources (2 for fluorescent use and one for

dark-field analysis). It has a dual-band emission filter.

At present only Via1-Cassette is used the NucleoCounter NC-200.

A number of different protocols can be run on the NucleoCounter NC-200. All of them are

documented in application notes describing the procedure to be used for the protocol. The

basis of all these protocols is to provide the operator with a simple set-up to perform an

extremely advanced analysis in a very easy and flexible manner so that all personnel can

be trained in a very short time.

The NucleoCounter NC-200 runs together with the NucleoView NC-200 software, which can

be installed on a PC having Windows XP (Service Pack 3) or Windows 7 operating systems

and USB 2.0 ports. See the Help Menu in the NucleoView NC-200 software and the

“Install_Guide.html” file on the supplied USB Flash drive for the list of PC Specifications.

2.1.2

The Cassette fixture.

The cassette fixture holds the cassette during analysis in the instrument. Refer to section

“7.1 Cleaning” for guidelines on how to clean this fixture if required. A stepper motor

moves the cassette piston after the RUN button has been activated.

2.1.3

The RUN Key and LED indicator.

On the front of the NucleoCounter NC-200 there is a RUN button. Activating this will run

the last performed type of analysis on the instrument. In this way it is easy to perform a

number of identical analyses in a routine manner.

On the rear of the instrument, a USB data connection (rev 2.0 or higher), a DC Power

Connection and a DC fuse holder can be found. Please take care of these areas and keep

them free of liquids and foreign objects at all times.

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