ChemoMetec NC-200 - Manual (Page 21)

2 Cell counting with the NucleoCounter NC-200
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2.2 The Cassette
Figure 2 The Via1-Cassette. Here fluorescent dyes AO (acridine orange) and DAPI (4',6 -
diamidino-2-phenylindole) are immobilized in the first part of the flow system.
2.2.1 Loading the Cassette
The cassette is loaded by gently pressing the white piston down to the top of the cassette,
which creates a partial vacuum in the flow system. The tip of the cassette must be
immersed into a sample mixture when pressing the piston, resulting in sample being
loaded into the flow system. Approximately 60µl is loaded into the flow system filling the
first part of the channel in the cassette. The liquid is transferred to the measurement
chamber when the cassette is inserted in the NucleoCounter NC-200 instrument and an
analysis is initiated. Please note that approximately 100 µl in a normal micro centrifuge
tube is recommended to reduce the risk of air being loaded into the system.
Figure 3 Loading of the Cassette