Hoefer SE1200 - Manual (Page 15)

Thick Gels
(Gel thickness >0.75 mm and acrylamide >10% T)
1
Fix, stain and destain the gel according to your
preferred protocol. Use a final destaining solution of
7% acetic acid and 5% methanol.
2
Final soak solution.
Coomassie Blue. After the final destain, soak the gel
in a solution of 35% ethanol and 0.5 – 2% glycerol for
1 – 2 hours. (Ethanol dehydrates the gel, which mini-
mizes cracking during drying. Do not place the gel in
the ethanol solution longer than 2 hours, as ethanol
will begin destaining the gel.)
Silver stain. Add 0.5 – 2% glycerol to the final
water rinse.
Fluorography. Follow the manufacturer’s instructions.
For fluorography agents EN
3 HANCE and sodium
salicylate, add 0.5 – 2% glycerol to the final cold water
precipitation step.
3
Load the gel onto the drying frame as described on
following page.
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p6
Note: Unless gel cracking is a
recurring problem, prevent gels
from becoming tacky by using
2% glycerol or less in the gel
soaking solution.
Note: If you soak gels in a
solution of ethanol and glycerol
you can dry the gels after a
2-hour soak.