Ocean Optics SpectraSuite - Manual (Page 148)

A: Experiment Tutorials
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6. If you have not already done so, place a sample of the solvent into a cuvette to take a reference
spectrum. You must take a reference spectrum before measuring absorbance.
Note
Do not put the sample itself in the path when taking a reference spectrum, only the
solvent.
7. Click the Store Reference Spectrum ( ) icon on the screen. This command merely stores a
reference spectrum in memory. You must click the Save Spectra ( ) icon on the toolbar to
permanently save the reference spectrum to disk.
Then click . The fourth page of the wizard appears.