Ocean Optics SpectraSuite - Manual (Page 159)

A: Experiment Tutorials
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At any time, you can click to reopen the setup wizard and change the
parameters for the concentration measurement.
Transmission Experiments
Transmission is the percentage of energy passing through a sample relative to the amount that passes
through the reference. Transmission mode can also display the portion of light reflected from a sample,
since transmission and reflection measurements use the same mathematical calculations. We express
transmission as a percentage (%T

) relative to a standard substance (such as air). SpectraSuite calculates
%T

(or %R

) with the following equation.
S

- D

%T

=
R

- D

x 100%
Where:
S

= Sample intensity at wavelength 
D

= Dark intensity at wavelength 
R

= Reference intensity at wavelength 