Ocean Optics SpectraSuite - Manual (Page 164)

A: Experiment Tutorials
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Note
If possible, do not turn off the light source when taking a dark spectrum. If you must turn
off your light source to store a dark spectrum, allow enough time for the lamp to warm up
again before continuing your experiment.
You must take a dark spectrum before measuring reflection.
6. Put the sample in place and ensure that the light path is clear. Then, take a reflection
measurement by clicking on the Transmission ( ) icon on the toolbar or selecting Processing |
Processing Mode | Transmission from the menu (since the mathematical calculations used to
calculate transmission and reflection are identical).
7. If you received calibration file with your reference standard, click the Non-unity Correction icon
( ) on the toolbar. The Select Reference File screen appears.
8. Browse to the location where you stored your reflectivity standard files. Then click the Apply
button.
The reference information appears on the Select Reference File screen.