Ocean Optics SpectraSuite - Manual (Page 154)

A: Experiment Tutorials
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d. If desired, enter the name and concentration units for their compound in the indicated fields
(this step is optional).
e. To generate a calibration, enter a known concentration in the Concentration field, and either
manually enter the corresponding Absorbance or place the corresponding cuvette into the
cuvette holder and click Use Last Scan to measure the absorbance directly. Use Notes to
enter any comments.
f. Click Add Sample to log the concentration vs. absorbance point. It will appear on the
Regression plot in the bottom of the window, and in the table at the top.
g. After entering a few points, a regression line will be computed through the concentration
points. This indicates that sufficient points have been sampled for concentrations to be
estimated from unknowns.