Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 125)

x
MAP Technology Protocols
PN 89-00002-00-150 Rev. A 9 - 7
amines. If any of these agents exist in the protein preparation,
remove them through dialysis or chromatography.
3. During activation, centrifuged xMAP microspheres may form a
sheet-like layer on the side of the microfuge tube. This is normal.
4. For best results, we recommend using microcentrifuge tubes
from USA Scientific, Inc., catalog number 1415-2500. To order,
call 1-800-LAB-TIPS.
One-Step
Carbodiimide
Coupling of
Oligonucleotides to
xMAP Carboxylated
Microspheres
Introduction
These protocols are a general starting point for developing assays.
All assays should be optimized for your reagents in your specific
application. Methods that give you the best results start with these
guidelines and are modified for your specific needs.
Equipment
• Vortex
• Sonicator bath
• Micropipetters: (1 µl - 1000 µl)
• Microcentrifuge
• Timer
• Analytical balance
Materials
• xMAP carboxylated microspheres—LIMIT EXPOSURE TO
LIGHT!
• Amino-substituted oligonucleotide (See Technical note 1)
• Microcentrifuge tubes: 1.5 ml, polypropylene
• Pipetter tips: 1 µl - 1000 µl
• EDC:
1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride,
Pierce Chemicals
• MES:
0.1 M 2- (N-morpholino) ethane sulfonic acid pH 4.5, filter and
store at 4°C.
• Tween 20 (0.02% v/v)