Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 43)

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MAP Technology Installation
PN 89-00002-00-150 Rev. A 4 - 5
For a filter bottom plate—stack three of the larger (5.08 mm
diameter) alignment discs together and place them into well A1.
For a half-volume plate with flat-bottom wells—stack two of
the smaller (3.35 mm diameter) alignment discs together and
place them into well A1.
For a round-bottom (U-bottom) plate—stack two of the
smaller (3.35 mm diameter) alignment discs together and place
them into well A1.
For a plate with conical wells—place one alignment sphere into
well A1.
4. Click Eject.
5. Place the 96-well microtiter plate on the Luminex XYP
instrument plate holder with position A1 in the top left corner.
6. Click Retract to retract the plate.
7. Loosen the probe adjustment screw on the probe holder one-third
to one-half turn. Pull it upward until it touches the top of the
adjustment slide. Tighten the probe adjustment screw.
8. Click Options on the toolbar.
9. Click Setup XY, then click Setup. A dialog box appears.
10. Click Test to lower the sample probe down.
11. Loosen the probe adjustment screw 1/3-1/2 turn.
12. Gently push the sample probe down until it just touches the top
of the alignment discs or sphere.
13. Tighten the probe adjustment screw.
14. Click Test to raise the sample probe, then click OK to close the
dialog box.
15. Replace the clear plastic shield that covers the sample probe
area.
Note: To avoid adjusting the
sample probe too low, open
the Luminex XYP door as
you begin adjusting the
probe height. The plate
should not bounce up or
down as you lower or raise
the sample arm. DO NOT
PUT YOUR HAND INSIDE
THE LUMINEX XYP!