Techne TC-3000G User Manual

Page 48

Advertising
background image

GRADIENT CALCULATOR

It is not possible to view the actual temperatures of each column in the progam or while the unit is
running. Therefore for any set gradient, the gradient calculator can be used to calculate the temperatures
for each of the columns and display them on the screen. This allows the user to determine the optimal
amplification conditions for the assay.

Programs

[0]

Information

Configuration

Gradient calculator

[ , to select ]

Gradient calculator

Temp 50.0°C

Grad 5.0°C

Column 1

47.5°C

[ , to select ]

Gradient calculator

Temp 55.0°C

Grad 5.0°C

Column 1

47.5°C

[ edit temperature ]

The gradient calculator field on the front
screen allows a user to input a temperature
and gradient range. It then calculates the
temperatures for each of the columns.

From the front screen scroll down to Gradient
calculator.

Press "Enter"

Press "Enter" again to edit the
temperature and gradient range.

Press "Enter" when finished and use the
"Up Arrow" and "Down Arrow"
keys to scroll through the list of column
temperatures.

Gradient calculator

Temp 55.0°C

Grad 10.0°C

Column 1

50.5°C

Column 2

51.6°C

Column 3

53.2°C

Column 4

55.2°C

Column 5

56.9°C

Column 6

58.2°C

Column 7

59.1°C

Column 8

59.6°C

[ , to select ]

46

Advertising