Eppendorf In situ Adapter - Manual (Page 4)

10
1 General information
The in situ Adapter for the Eppendorf Mastercycler ® and the Eppendorf
Mastercycler
®
gradient enables in situ PCR reactions to be carried out on
a specially developed carrier system. This allows the Mastercycler ® and
the Mastercycler
®
gradient to be used for the conventional PCR technique
in different tube systems as well as for the in situ technique.
The in situ Adapter is a high-temperature-conductive metal carrier, which
can accommodate up to four glass slides. To ensure optimum temperature
control of the samples, the entire carrier system also has a detachable
covering hood. This covering hood is transparent and double-walled,
which allows access to the sample at all times and also prevents heat
loss.
The adapter is extremely easy to handle: The carrier system is positioned
onto the thermoblock of the Mastercycler
® or Mastercycler ® gradient. The
samples are placed on up to four object carriers, fixed into place and
sealed hermetically prior to the PCR. Eppendorf
in situ Frames form the
ideal sealing system for the in situ PCR and are available in different sizes
(volumes) (see "Ordering information"). The transparent covering hood is
then closed and the PCR may be started.
Thanks to the wide selection of different tube types, the software is
specially tailored to suit the
in situ Adapter, which guarantees the high-
quality heat transition necessary for optimal results.
The in situ Adapter must be set up and used in accordance
with the operating manual provided in order to protect both the
user and the device. The instructions in the operating manual
of the Mastercycler
® or Mastercycler ® gradient must also be
observed.
When operating the device, please observe the instructions
contained in the corresponding applicational regulations!
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