Eppendorf In situ Adapter User Manual

Page 7

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13

3.2 Technical description

The glass slides are placed onto the four contact surfaces of the metal
carrier:

3.3 Programming

The Mastercycler

/ Mastercycler

gradient is programmed in accordance

with the operating manual for these devices. The

in situ

Adapter is treated

by the Mastercycler

and Mastercycler

gradient like all other tube types

(i.e. for the

in situ

PCR,

"in situ"

is selected instead of the tube types

"0.2 ml", "0.5 ml" and "plate"). Specific settings for the adapter are thus
selected.

During such programming, please observe that the temperature of the
sample (TUBE) is regulated. The lid heating

switches off automatically

(corresponds to LID=0°), irrespective of the entry in the LID field.

Up to four glass slides can be attached to the carrier

FILES...

Edit UNNAMED

Edit

CNTRL

TUBE

Load

LID=0°

Standard

NOWAIT

AUTO

New

1

T=94.0°

00:02:00

Delete

2

T=94.0°

00:00:15

UNNAMED

3

T=44.0°

00:00:15

– –/22.0°

10:22:06

Temperature regulation is set to TUBE

and the temperature control of the lid switches off

B_in_situ_Adapt_e.fm Seite 13 Dienstag, 2. August 2005 1:42 13

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