Leica Biosystems M80 Measuring - Manual (Page 6)

6 Measuring – User Manual
The graticule is only magnified by the eyepiece.
The total magnification of the specimen does however
depend on the lens, magnification changer, eyepiece and
additional tube possibly used (e.g. coaxial lighting sources)
and changes whenever these factors are altered.
When calibrating with the stage micrometer, the value of
an interval on the graticule is defined in relation to the
specimen magnification. The true dimensions of a
specimen can only be established once the calibrated
value has been defined.
… if you only undertake relative measurements
or comparisons.
… if you need absolute measurement values.
The calibration value of each of your optics/
magnification combinations used only has to be
established once. Keep a table of the appropriate
calibration values (refer to example on page 12).
When working with instruments with engageable
zoom settings (Leica MZ6, MZ7
5
, MZ9
5
etc.) or
adjustable stops (Leica S6 models etc.), the specific
magnifications can always be re-produced
exactly (refer to directions for usage for the stereo-
microscope).
When using instruments with zoom magnification
changers without engageable setting stages,
inaccuracies could arise when again undertaking
settings. If a high degree of measurement precision is
required, you should therefore undertake calibration
whenever changing magnification.
Why calibrate?
You do not have to
calibrate
You have to calibrate
Only calibrate once
Engaging zoom
settings
Calibrating graticules