Transmitted-light base tl bfdf: operation – Leica Biosystems M205A User Manual

Page 79

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Leica M series

Manual

79

Light intensity control

Please observe the user manual—in
particular, all safety regulations—from

the manufacturers of the light guide and cold
light source.

Switch on the cold light source according to

the manufacturer's user manual and adjust
the brightness.

Transmitted-light control

The TL BFDF transmitted-light base has a poten-
tiometer that switches the light from "bright
field" to "dark field".

Bright field

Bright field is suitable for examining translu-
cent objects featuring contrasting structures.
The object is directly illuminated from below
and is seen in its natural colors against a bright
background.

Turn the control as far as it will go towards

"BF" ("bright field").

Dark field

In dark-field illumination, a ring illuminator is
used in such a way that the direct light
does not reach the objective without a speci-
men. Only the structure of semitransparent,
opaque objects disperses the light, making the
object visible against a dark background.

Turn the control as far as it will go towards

"DF" ("dark field").

Transmitted-light Base TL BFDF: Operation

Fingertip with bright field illumination

Identical subject with dark field illumination

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