Leica Biosystems M205A - Manual (Page 86)

Leica M series Manual 86
Dynamic relief contrast
Suitable for semitransparent and transparent
specimens.
By tilting the deflection mirror slightly, you can
move the gaps over the entire field of view and
quickly toggle between positive and negative
relief images. The dynamic effect makes it easy
to distinguish phase structures from amplitude
structures.
Constraints
The relief methods provide good results from
mid-zoom to high magnifications and with 1Ч,
1.6Ч and 2× magnifications. In the lower zoom
half and with weaker specimens, the object
field may not be uniformly illuminated.
We recommend using the transmitted-light
base with 1× or higher objectives, and not
objectives with a long focal length.
TL RCI™: Relief Images (continued)