Leica Biosystems M205A - Manual (Page 26)

Leica M series Manual 26
Objective Nosepiece – Adjusting Parfocality
The following procedure only has to be carried
out once. Afterwards, both objectives are parfo-
cal, meaning that the specimen remains in focus
when the objective nosepiece is rotated.
This procedure must be repeated if you replace
either of the two objectives with another.
The following example assumes the combi-
nation of the 1× and 2× planapochromats. If
you are using another objective combination,
replace the 2× objective in the description with
the objective with the stronger magnification.
Preparation
Open the iris diaphragm. ★
Set the dioptric correction of the eyepieces ★
to "0".
Adjustment
1. Rotate the 2× objective into the beam path
and set it to the lowest magnification.
2. Focus on the specimen.
3. Rotate the 1× objective into the beam
path.
4. Turn the objective on the thread in both
directions until the specimen appears
sharp.
5. Toggle to the 2× objective.
6. Select the strongest magnification and
refocus until the specimen appears abso-
lutely sharp.
7. Toggle to the 1× objective.
8. Turn the objective on the thread in both
directions until the specimen appears
absolutely sharp.
By means of zooming, check that the behavior
of the objective is parfocal. Repeat the check
with the other objective. If it is not parfocal,
repeat the procedure.
9. Tighten the locking screws.