Leica Biosystems M205A - Manual (Page 84)

Leica M series Manual 84
TL RCI™: Methods in Transmitted Light
Vertical bright-field illumination
Suitable for stained amplitude specimens with
sufficient contrast.
The light beams are deflected vertically through
the specimen. This results in an accurate bright
field with maximum brightness.
Inclined transmitted light
Suitable for semitransparent, opaque speci-
mens such as foraminifera and fish eggs. Move
the deflection mirror until the desired data are
visible.
Single-sided dark field
Suitable for fixed specimens and fine struc-
tures.
The flatter the angle at which the light beams
are deflected into the specimen plane, the
darker the substrate appears. A dark field-like
transmitted light is created. Outlines, fine edges
and structures are bright, in contrast with the
dark background, through diffraction of the
light beams on the dark background.