UVP Life Science - Manual (Page 106)

LS Software User Guide
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Dynamic Integration
Used when the integration time offered by the camera is not enough for a long exposure. In this mode, LS
software does "stacking" of frames, i.e., it adds the corresponding pixel values of first image-frame to the
next. This compensates for a low light limitation, by making dim areas brighter with increasing number of
images. [Stacking replaces the first image after the second is captured and pixel values added to it from
the first.] UVP cameras typically have a long exposure time capability, which would be more than enough
for most samples. If, however, the application’s requirements go beyond what is available, then this
feature offers a software solution.
Switch to the ’
Dynamic Integration’ option and click on ’Dynamic Integration’ button to bring up the
following window:
• Auto Exposure: With this box checked, the stacking automatically stops once the image gets
saturated.
• Total Time Exposure: Select this radio button to have a greater control over how many images
to capture, and for how long each one should be exposed. Interval Time is simply the division of
total time by number of images. Uniformly exposed images are stacked.
• Current Black Level: Stacking frames also stacks noise along with it. To clip noise from each
image before stacking, it is important to set the black-level. Value of the darkest pixel in the image
(which will typically be noise) should be set inside the spin-box. Pixel values less than this value
are not recorded or clipped during acquisition and before stacking.