UVP Life Science - Manual (Page 132)

LS Software User Guide
118
Background Correction
Background Correction Options
Background correction is necessary to account for possible variable illumination or overexposure during
image capturing, the software offers options to apply mathematical background correction. These options
generally remove background "noise" and elevated levels of pixel intensity due to excess exposure,
highlighting data.
Note: Before selecting to correct the background in the image, first find lanes and bands. To use the
background correction options, select 1D Analysis > Lane Profile > Background correction options.
The software offers these background correction options.
• No background correction
• Straight line
• Joined valleys
• Rolling disc
• Area between the lines
No Background Correction
Selecting this option on the menu leaves the image uncorrected for overexposure, "as is."
Straight Line
Selecting this option tells the software to place a straight (but not necessarily horizontal) line under
the lowest points at the beginning and end of each lane. The software removes the area of the
graph under the straight line, so that all remaining values are emphasized. Straight line correction
tends to correct the well for overexposure, and for variable illumination that is focused on an edge or
corner of an image.