Waters ACQUITY UPC2 Torus Columns User Manual
Acquity upc, Torus columns, Care and use manual
[ CARE AND USE MANUAL ]
CONT ENTS
I. INT RODUCTION
 
II. GET TING START ED 
a. Safety Considerations
b. Column Connectors and Installation
c. eCord Installation
d. Column Equilibration
e. Initial Column Efficiency Determination
f. VanGuard Pre-Columns
 
III. COLUMN USE
a. Sample Preparation
b. Solvents
c. Additives
d. Pressure
e. Useable Flow-Rate Ranges and Expected ACQUITY UPC
2
System Pressures
f. Band Spreading Minimization
 
IV. T ROUBLESHOOTING 
 
V. COLUMN CLEANING, ADDITIV ES, 
 
AND STORAGE
a. Cleaning
b. Effects of Additives
c. Aging
d. Storage
VI. INT RODUCING ECORD INT ELLIGENT 
 
CHIP T ECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
VII. ACQUIT Y UPC
2
COLUMN FAMILY
ACQUITY UPC
2
Torus Columns
I. INT RODUCTION
Thank you for choosing an ACQUITY UPC
2
®
Torus™ Column.
Torus Columns are specifically designed to use the complete 
range of capabilities of the ACQUITY UPC
2
System to achieve fast,
robust achiral separations. ACQUITY UPC
2
Torus Columns simplify
the method development process with four completely new and 
innovative 1.7 µm chemistries for UltraPerformance Convergence 
Chromatography.™ These columns are designed for excellent 
peak shape that eliminate or reduce need for additives, coupled 
with added selectivity for a wide range of compounds and 
improved robustness. 
These columns are based on new bonding technology used to 
create an adaptive selectivity platform. Starting with the ethylene 
bridged hybrid (BEH) particle, the patent pending bonding consists 
of a two stage functionalization of chromatographic particles. 
The first stage controls retention characteristics and is applied 
to each sorbent. The individual second stage selectors impart 
the observed selectivity and control peak shape.