Waters Dye QCRM Kit - Manual

Dye Quality Control Reference Material Kit 1
[ CARE AND USE MANUAL ]
CONTENTS
I. INTRODUCTION
a. MS-Directed Purification
b. The Purpose of a QCRM
c. What Affects Your QCRM Results
II. STORAGE AND STABILITY
III. RECOMMENDED USAGE OF THE DYE QCRM
a. Testing System Performance
b. Performing the Delay Timing Test Manually
c. Performing a Chromatography Test
IV. TROUBLESHOOTING
V. ORDERING INFORMATION
DYE QUALITY CONTROL REFERENCE MATERIAL KIT
I. INTRODUCTION
The Quality Control Reference Materials (QCRM) portfolio is a unique
collection of standards and mixtures. These products allow users
to evaluate and benchmark the chromatography system before
analysis of critical material. The products in the portfolio are all
precisely formulated based on the expertise of Waters scientist.
The AutoPurification ™ Dye QCRM contains 3 compounds including
Thionin, Thioflavin T, and Crystal Violet.
This standard mix is used to confirm the benchmark performance
of a Preparative/Purification System through fraction collection.
This particular QCRM is precisely formulated to allow the user to
verify correct fraction collection. This test is highly valuable to
perform upon first use/installation, and any time the system
has not been used or has had any changes. These compounds were
vigorously tested and evaluated and chosen because they provide
the following advantages:
• Well-separated
• Easily visually identified
a. MS-Directed Purification
The Waters MS-directed autopurification system uses the Waters
SQD2 Mass Detector as its primary detector.
UV-Directed System MS-Directed System
PC with MassLynx and
FractionLynx software 2767 sample
manager
2545 binary
gradient module
Bottle
tray
2489 UV
detector
System fluidics
organizer
PC with MassLynx and
FractionLynx software 2767 sample
manager
2545 binary
gradient module
515 makeup pump
(not shown)
Bottle
tray
2998 PDA
detector
SQD2
detector
System fluidics
organizer