Waters MetID Small Molecule Standard Mix - Manual

MetID Standard Mix 1
[ CARE AND USE MANUAL ]
CONTENTS
I. INTRODUCTION
II. STORAGE & STABILITY
III. RECONSTITUTION OF THE STANDARD
SOLUTION FOR TESTING
IV. EXAMPLE OF USING OMEPRAZOLE STANDARD MIX
V. ORDERING INFORMATION
VI. REFERENCES
MetID STANDARD MIX
I. INT RODUCTION
Omeprazole Metabolite Mix is used to test and demonstrate the
Waters UNIFI Application Solution for Metabolite Identification.
The standard was designed to both test and confirm LC and MS
performance but also to test and demonstrate the UNIFI system
solution. Omeprazole is a commonly prescribed medication used to treat
acid reflux causing heartburn (known clinically as gastroesophageal
reflux disease, GERD). When the compound is dosed in vivo in human,
two of the commonly detected metabolites which are formed in
human are 5-hydroxy omeprazole and omeprazole sulfone.
Omeprazole Sulfone
Omeprazole
N
N
H
N S
O
O
O
5-Hydroxy Omeprazole
N
N
H
N S
O
O
OH
O
N
N
H
N S
O O
O
O
Figure 1. Structures of Omeprazole, 5-Hydroxy Omeprazole, and Omeprazole Sulfone
Using Waters ACQUITY UPLC ® BEH column chemistry, the generic
gradient method can be used to analyze and effectively separate the
standard into its parent and 2 authentic metabolite standard components.
The standard mix stock solution comprises Omeprazole, 5-hydroxy
omeprazole and omeprazole sulfone at concentrations of 10, 1 and
0.1 µg/mL respectively.
II. Storage and Stability.
The lyophilized powder is shipped at ambient temperatures, in brown
amber vials in foil packaging. It is highly recommended that upon
receipt, the standard should be stored frozen and thawed before use.
Once reconstituted the sample should be used immediately for
best results. Omeprazole has been reported to be unstable in the
presence of continuous exposure to UV. Care should be taken to prepare
samples and standard curves out of direct light whenever possible. If