Waters MassPREP Micro Desalting Column - Manual

CONT ENTS
I. INT RODUCTION
II. CONNECTING T HE MASSP REP MIC RO
DESALTING COLUMN WIT H T HE CHOSEN
LC SYST EM
III. LC /MS SYST EM CONFIGURATION TO
MINIMIZE MS SOURC E CONTAMINATION
IV. USING MASSP REP MIC RO DESALTING
COLUMN TO MINIMIZE P ROT EIN CARRYOV ER
BET W EEN INJECTIONS
V. GUIDELINES FOR USE
VI. RECOMMENDED STORAGE
I. INT RODUCTION
Mass spectrometry is a powerful tool for intact protein analysis.
However, the quality of analyses can be compromised by salts in
the sample that interfere with ionization and/or form adducts with
the investigated proteins. It is, therefore, important to remove or
significantly minimize the introduction of extraneous salt ions into
the mass spectrometer. The use of on-line, desalting devices can
efficiently perform this function with minimal sample handling.
Waters MassPREP™ Micro Desalting Columns (2.1 x 5 mm, P/N
186004032) contain a polymer-based, reversed-phase packing
material (20 µm particle size, 1000 Å pore size) that can be used
to effectively desalt protein samples prior to mass spectrometry.
The phenyl phase material contained in these on-line devices
successfully retains proteins allowing the salts to be washed
to waste prior to elution of the desalted protein into the mass
spectrometer. With an optimized LC/MS method, cycle times as low
as 4 minutes are achievable. These on-line devices, when used with
an appropriate LC system injector and injector wash solution, can
be used for the sequential analyses of different samples.
II. CONNECTING THE MASSPREP MICRO DESALTING
COLUMN WIT H T HE CHOSEN LC SYST EM
The MassPREP Micro Desalting Column can be used with an
appropriately performing LC system to perform the desalting
functions so long as proper fittings/tubings are used to make
connections. However, optimized performances are achieved
only when the cartridge is connected to a Waters ACQUITY or
nanoACQUITY UPLC system. For the use of the cartridge with
ACQUITY or nanoACQUITY, pre-assembled tubing kits (Intact Mass
Tubing Kit, p/n 205000548) are provided to connect the column to
the UPLC systems and mass spectrometer.
There are two configurations to connect the cartridge into the LC
fluidic path. The inlet of the cartridge can be directly screwed onto
the Valco
® UPLC injection valve with no tubing connection.
MassPREP Micro Desalting Column
[ CARE AND USE MANUAL ]